Abstract

Olive oil deodorized distillates are rich in 3-5% tocopherol and 8-10% squalene. This study used the saponification technique, one of the pre-separation techniques, to purify tocopherol and squalene, which are found in high amounts in deodorized distillates. The saponification process removed 70% of free fatty acids, and 9-10% tocopherol and 17-18% squalene were purified. Tocopherol and squalene obtained after the saponification process were enriched using semi-preparative chromatography. 6 different fractions were obtained. While the second fraction contained high amounts of tocopherol and squalene, it was seen that the third fraction contained squalene and other compounds separated from tocopherols. As a result, 31-32% tocopherol and 36-37% squalene were obtained.

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